{ "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "FrameKey-0-0-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2fc08dc3-d8a9-41c2-af6d-55f5be4ebd84", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2053616, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2a3d7bfa-4534-427f-9ade-cc36ea7e5a16", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2054083, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d8839195-d18f-4f9b-a247-9fd83a432466", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2054551, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "da13975d-769e-4248-8ea5-5bc74046ca32", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2055017, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eca9c164-bbda-429c-87fe-d96313239ead", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2055483, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7340c9f5-e6bf-4f95-a672-be4eef09e39e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2055950, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e8f0c2e9-8645-4e6f-b06e-c034bc883b93", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2056416, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "343b8b98-ecbe-44c8-8597-0e1c5bf1d098", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2056883, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c8922ff9-6694-4d57-8590-74ee29f7eb74", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2057351, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "83289748-f67e-471d-aee9-ce8bae463d1e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2057817, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ca47ade7-c9ed-4097-bd36-0d3a3f0aef74", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2058285, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a4fdabeb-c368-437f-83f4-fb3f381343a2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2058752, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3615db82-6117-48e1-9f16-501f0ae8d214", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2059220, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b07cf935-df67-4273-a943-e080902f22d2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2059688, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "378ebc04-60ca-4441-9202-3e353440f094", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2060157, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "55328bca-4486-4672-9aca-c11668a3ffdc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2060625, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "31e43db8-967a-45b7-a022-67baa029f518", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2061095, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fd1699d6-6086-4e60-8f77-9af90541f3ff", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2061563, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d013ec68-38fa-43ac-aac4-545c6913c933", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2062041, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b7e58b85-773a-4305-b1e1-c02dce840065", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2062510, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "de6db95f-2345-404d-8399-a93a2a3c024c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2062978, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4950fdac-8f6c-4345-b5b2-6c2f896ebf5e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2063445, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ee220597-6f67-431a-8d9e-d3375e27bbf6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2063915, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1694aabe-5c19-4735-b6cb-83177b32ab92", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2064383, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "984f6649-b7dd-4ac7-b59a-d698ca5d7fcd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2064851, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "70714ea4-026b-444c-839a-a44be4243364", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2065319, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a2d160e9-c91f-4594-9ea3-49bcb5b8b47f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2065787, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b16dd108-fdd2-4b62-8133-03e236aa4bb3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2066274, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4afac0a4-266d-4998-a34c-66d50573409e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2066742, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3cb623ac-3571-4ed2-b1af-621af4fa7b90", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2067211, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "98b16d51-7793-49f0-9eda-acb8f2cacae1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2067678, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:29 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "01c22e56-f61a-4aa1-8735-8756ffb000a7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2068146, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d3085700-4656-4f37-820d-8cbd572261f3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2068615, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "977550f2-305e-43a5-a1ca-340d2f8958e5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2069082, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dffb1e48-abca-4a07-be71-7bd7e1450cb6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2069550, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e8a519be-eda7-4c51-8ac7-b49c2a337f30", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2070026, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ca969028-f759-45e9-9d19-833ccdc89c48", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2070521, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7e0a113f-297c-4875-97e1-81fa24df1644", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2070989, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0830e79b-ec39-44b9-8ed9-beba36a4008a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2071459, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dadf3fb5-5189-4340-8e94-eebfa3337c4f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2071927, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-0-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "5.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 380, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4581d51a-71fa-4e5a-9dd4-a37641454610", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "CFP/YFP", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "565/24", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "5.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "520", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2072412, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "380.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-G_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-G", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 0, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "589894e9-473a-4c65-8999-b2c3ee6d7251", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2073409, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8ab314a6-9133-4fa3-9551-4b5b6c2deaf8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2073657, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d076527c-c961-4b83-9eee-0a03234b1ce5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2073907, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5559f07c-2e87-434a-b40d-0962285b9bef", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2074153, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ef174c9d-0c1a-43d2-b2eb-e153b66ba55f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2074400, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fb3390a3-6ec8-44aa-acf5-8dfa2275df39", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2074647, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a49b3749-4b6f-4616-8755-0643ea2a130e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2074895, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c3e1cc24-f34a-499e-88c1-399495554fa4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2075148, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a1d4f2be-e3af-4b8a-b997-16d7fc10aba0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2075411, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c49b6aa1-b4cc-43cf-a3c2-272fb3586736", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2075658, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "22e64a3e-ca9c-4166-a7d2-8a91b78823d4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2075906, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3ad9de1f-a06c-4ba8-9c4c-fe2faf0ddec3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2076152, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f37d4719-97ad-4836-9575-1c941326b900", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2076400, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1ac81704-0f4c-4a14-877b-e32b423a891b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2076646, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "392ebea3-4c7b-45c2-a18e-6ece417310c8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2076892, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "64f37fb9-d2b9-4b82-9299-62d76eefe4d4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2077138, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e4b3d24a-4772-41bb-a9d1-997bd0cff305", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2077385, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "065529e6-6758-45f4-8fe7-c8cf6f807a6a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2077645, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e4301aec-4eca-40ee-8e01-0564009ffa38", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2077892, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6e9c2ab4-1b0c-47e9-9a2f-ab15d7c80195", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2078138, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3c1bb6a8-d25b-497d-bd7a-569ff89622a3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2078386, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "29fc5edc-bbb9-4096-8b35-05373547555f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2078634, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "09011fda-2b34-4d5d-97cd-d718281a3628", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2078882, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6fe95be1-8121-4603-b712-b8b51d6b08c7", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2079128, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "60ce6070-c811-43b6-8b34-0692fa94f8dc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2079397, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "96a8489f-170d-440a-bfd4-da58fa235787", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2079647, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b316c9e4-14e1-45b4-b86d-33fad66a5783", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2079894, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "11881e2a-89f2-4b8d-91fa-5198b14944cc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2080142, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cbaba632-f1d8-4a93-aa9f-2b1ab00fa1c5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2080388, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c6ad2739-e3e8-43ca-9e5b-bfae94994986", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2080636, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a793340f-2297-44ce-967a-4b3163e48f96", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2080883, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "58ffcac5-37e8-46a6-93ab-4fbbd158d290", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2081132, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "98bc99cd-02b1-41ce-9265-af8c1ce6d858", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2081379, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ae430d48-3ae4-4e07-8871-fb0fe20d286b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2081634, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d39bb212-2de6-45c6-8ded-a883770cc4ce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2081882, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3281c833-e5b2-43fe-88f9-a83ce6906b08", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2082130, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6a8329be-08de-4b36-a864-20255084e432", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2082377, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "909ae92e-06a0-4860-bd67-c142be8ba25c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2082624, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "57ef4e78-b1bb-47ce-9892-047fd0144bdc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2082872, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "805b0c21-7731-4fb8-9b98-c7ab89495c35", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2083119, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-1-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 160, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1d235e6a-7b1c-4813-8de8-4ef109476dbd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "585/11", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "555", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2083365, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "160.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-T_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-T", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 1, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "5.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "5.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ad5c5789-f7e4-459f-8b91-df236120dbbe", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2084514, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7fb9571c-8d62-4a55-ae74-c4fabd4369c6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2084961, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "61f00fbe-ed1c-4372-a7e4-adf495c33be9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2085414, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "20f1d117-991d-4296-b0cb-914be7b16711", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2085862, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "bc345f32-c0fc-4f8b-95bc-917cff2c99c9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2086309, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "cf95a8a4-94b0-4155-942a-6b351d05d73c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2086757, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:48 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "841159bf-9bdc-463e-9116-6910358c8d13", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2087205, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7c0bd1d6-78fc-41db-85ba-3c38f5c2b25f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2087672, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:49 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3be5777e-959d-4e5d-98f8-c1efe01ffba5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2088119, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "eb931708-97bf-44a9-a5fd-a5b1f61ef47b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2088566, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:50 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d182e144-744c-4c5e-a594-a5e4951b1dce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2089015, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8d83d70d-b2d4-4df4-870a-9b7c52fefe30", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2089464, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:51 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0877b116-21f4-4564-bcbd-a638069b7f93", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2089912, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "1e52ee06-9d38-4a0a-8ae7-0ee3254af42b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2090360, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6fb5274d-a016-45f5-8ada-4fbe8802d4ce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2090808, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:52 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ef53991f-8840-4c09-a826-4cc54fafd8e9", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2091257, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2e498a11-8095-4b53-8e45-ffb4b1f9d189", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2091705, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:53 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f32737ad-5212-4bf8-8dd7-1230249effce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2092153, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "19628483-b0e2-4f6f-aca4-7a39758123cc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2092602, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:54 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "802a1f22-70e4-40c0-beeb-4e4b262f59cb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2093057, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b346b647-0643-48fa-a8b3-7e2dee204d28", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2093505, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:55 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "354bec10-3593-4c0b-b5b0-471fd7e7def4", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2093953, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2df7df10-530f-4f79-bf72-02bd50e4b3b5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2094400, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "50b656db-c4be-4452-b8b4-043bfad1f1f8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2094849, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:56 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3b58a17e-0b05-4d94-b628-d327b254267a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2095297, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8ed0f66e-6a50-480b-9fc9-c232e54aa83b", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2095745, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:57 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0cd62a45-3c53-48da-bb9d-ba4b2577bb0a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2096193, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "26fb82d1-9e49-40de-8aaa-9c88e96d7577", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2096641, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:58 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "beabf1a9-a633-4f66-a078-d622bdbf74d2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2097090, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6d1771b6-e98c-4110-86bf-fdb03af9ed39", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2097539, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:55:59 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "866b3b7b-4ec1-4c2c-96c3-6e346e928dd5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2097987, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5be10fa0-efe7-4d8b-a521-6e8239149ab3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2098437, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "2748998b-b6e8-40fc-858a-ce728c25f294", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2098885, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:00 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "df48cabe-3a4e-4bf5-9b78-842a6ab0c1b0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2099342, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b3e33c26-90ae-4ac8-8715-eb76e1ac29e3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2099790, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:01 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6c684b0d-dc32-458b-8a24-47b121956c9e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2100238, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "424355eb-b19d-41e9-9b3c-960148045da0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2100686, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:02 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "86aaca35-e220-4174-bc7f-cc77fbb772c2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2101134, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "38d4a550-3db5-445a-a998-89faa90c7537", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2101582, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:03 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c6fa8845-594a-44a8-991a-4d38b5b55463", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2102031, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-2-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 360, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "77d5bd0b-dca3-4888-9ec1-4abf08865a73", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "676/29", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2102479, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "360.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-A_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-A", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:04 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 2, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "961700a4-14df-484b-8ef1-441eb91dde57", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2103352, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:05 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "67f6b973-a1e2-455d-b51b-7ad4e7a53cb6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2103948, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "272d4587-91a5-468e-9626-2896c89e62b0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2104532, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:06 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0bc09ef6-d031-401c-9819-6089ffc0ff56", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2105118, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "936315d6-6b69-43a8-b50f-98e557da2cb6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2105711, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:07 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d57936d2-e0e3-45d0-b050-ec6b528c3a19", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2106297, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7005bcd9-75db-41e2-94f2-43dcca6c72ed", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2106883, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:08 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5a34abdf-794e-4f14-a022-78076936a850", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2107471, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:09 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4255a85f-8375-4a66-a89c-04843bbeb577", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2108057, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f7eacfea-9670-4b7a-b882-356cb9757ce6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2108643, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:10 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a2caaedc-616f-4471-b7dc-1127988a6f02", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2109230, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "49f6fd48-2363-45d4-82a6-936b2207b4f8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2109815, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:11 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9d251a00-80b7-4967-94b5-6c550fb3c614", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2110403, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:12 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "0da5f3cb-f0fe-432b-9246-b050217202da", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2110989, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4542c16d-4677-4a0e-9e44-579f55673bc3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2111576, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:13 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7a930733-71a1-4ac0-9450-79f51a71854f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2112165, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b4eb2a61-001b-461c-9af8-4a9d117333c3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2112754, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:14 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ab8f4d8e-6f53-42ec-bb8a-c934b8441e6c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2113340, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:15 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8552f4f3-245a-41fe-a322-340730b8eaa0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2113927, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "615e40c7-6eb2-45ca-a520-2fb917ead29e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2114513, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:16 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "6eb8dc2e-4907-42c3-b6c2-e95c8ade7672", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2115100, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dfd75a8b-3b6d-40d5-a00d-67921716bf3f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2115700, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:17 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f29bb8b8-e605-44d5-bb53-7485b94ce607", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2116288, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d6021198-b1ee-47b7-9f2b-4958f8408511", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2116876, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:18 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7160bb2e-beda-4144-b136-36eadd6e1522", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2117465, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:19 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "df604495-00d2-46f3-86ce-f57e6c7a9387", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2118051, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9c7c93e1-4617-4603-89a8-edbfcc380a4a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2118637, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:20 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d74ffa3d-64bd-4b7b-af3b-608f595687bb", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2119223, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "42d6b6f0-5036-4e2e-85d7-54679c2c4521", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2119811, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:21 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e5f15a45-47bb-4e97-b7c1-eb944c7745df", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2120408, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:22 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ef541971-e944-4608-a594-f168d54ddf6e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2120994, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4d529eb5-297c-4728-bc87-0c147595c62f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2121580, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:23 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ce725c24-f997-4668-ac64-5c83f0b882ff", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2122166, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f6f4c490-8c96-4492-ae05-9949e42f4b52", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2122754, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:24 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "66eb077c-a241-4d59-b1c2-b5b1c7229b10", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2123342, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:25 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "334544be-110e-4dc9-9f9d-4a7caa46867f", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2123930, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9edf6c66-8371-43cd-bfd8-b5b42c567f4e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2124529, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:26 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a9e0251e-89a4-4515-a51f-98fc5fdfa5e1", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2125115, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "31c83c07-fcb3-4aa7-b1f3-f90f44f08789", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2125701, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:27 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "471fbe62-d9a2-4b32-a06b-a72cefe6b622", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2126287, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-3-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 500, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e8056d5b-abc4-42c0-a0d0-082bbb6efdce", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "FF652-Di01", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "725/40", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "640", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2126873, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "500.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "5.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_Il-C_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "Il-C", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:28 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 3, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "5.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-0": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "406b602d-7aa9-442e-9663-6420f40d6ebf", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 0, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 0, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2127960, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_000.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 90, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-1": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "109bebd2-60a6-444e-9f6a-7ecfb54ce7e3", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 1, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 1, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2128393, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_001.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 91, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-2": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f64e79a8-ca72-40a2-a42b-2b7ea5abfd34", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 2, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 2, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2128809, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_002.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 92, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:30 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-3": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3f8de64b-7127-4c95-bca7-96eee0e6c38c", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 3, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 3, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2129256, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_003.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 93, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-4": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8a7b2b8e-0a8a-484f-8a1b-c69148bfa031", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 4, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 4, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2129689, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_004.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 94, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:31 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-5": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "539097da-e378-4766-bee2-c9f3f89240fc", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 5, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 5, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2130130, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_005.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 95, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-6": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e430ab0e-9b55-49ed-8c18-ab98beab32e0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 6, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 6, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2130584, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_006.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 96, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:32 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-7": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "870a3c6c-c7c8-4109-aab1-d81cf3979948", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 7, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 7, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2131016, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_007.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 97, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-8": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9757fecf-170b-4e6c-9d80-9bc84cadffda", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 8, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 8, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2131448, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_008.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 98, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:33 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-9": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e6bc0f92-d32d-4dcf-b872-d69ca65dca4a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 9, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 9, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2131916, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_009.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 99, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-10": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "f194a660-c820-4e2a-8c4f-0f50a09d63b8", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 10, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 10, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2132344, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_010.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 100, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-11": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "7c009c26-904e-4034-991b-d51d17f55285", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 11, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 11, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2132776, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_011.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 101, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:34 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-12": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "67991a66-b99d-41a4-98f0-3c4c5499e15d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 12, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 12, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2133192, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_012.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 102, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-13": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "fe293440-4588-4741-a0d3-388d4f02da68", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 13, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 13, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2133623, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_013.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 103, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:35 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-14": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "771cb448-5161-4020-8b38-108d019d3385", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 14, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 14, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2134040, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_014.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 104, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-15": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "8db75821-13d3-4181-9bbf-df948a0e4a05", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 15, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 15, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2134488, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_015.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 105, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:36 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-16": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "427e2901-37df-43ae-a6ab-515e9f8dada6", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 16, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 16, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2134936, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_016.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 106, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-17": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "245e4e35-70d3-4310-b430-9c956893a529", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 17, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 17, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2135381, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_017.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 107, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-18": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "dbc0e7a9-e439-4b90-8f9d-73f5c6b97f14", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 18, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 18, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2135815, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_018.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 108, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:37 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-19": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": -1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "3cb89387-9270-4bb1-939f-9978558aa327", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 19, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 19, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2136246, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_019.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 109, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-20": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 0, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "01a35740-00c7-4293-901a-ed27e0d4db36", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 20, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 20, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2136693, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_020.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 110, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:38 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-21": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 1, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "12dd0d2b-e0ac-47e3-9e88-190aeae46021", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 21, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 21, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2137143, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_021.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 111, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-22": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 2, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ec09595f-1057-4555-8fc7-b088bb12f74d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 22, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 22, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2137591, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_022.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 112, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:39 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-23": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 3, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "b6bc4def-f82d-4de7-a168-ffb5f44a49dd", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 23, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 23, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2138007, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_023.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 113, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-24": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 4, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "52ce8f51-2ae2-4ec8-8c0d-e653c2623a69", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 24, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 24, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2138457, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_024.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 114, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-25": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 5, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "4a576937-c740-42b4-a332-52812ec058e0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 25, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 25, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2138885, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_025.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 115, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:40 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-26": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 6, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "85a3a96f-e99e-4dd0-bf5b-70ade46f1453", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 26, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 26, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2139302, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_026.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 116, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-27": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 7, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "5f5b9f9f-95f2-49a4-9c22-78952f40a9ad", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 27, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 27, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2139751, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_027.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 117, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:41 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-28": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 8, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ff2ac4a7-38b4-43b5-88e6-2ad59e402548", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 28, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 28, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2140198, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_028.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 118, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-29": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 9, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "c79469ae-dfd3-4da6-97b7-d304bc95e804", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 29, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 29, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2140628, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_029.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 119, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:42 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-30": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 10, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "23c3c1b7-c896-478f-8688-1da6a18dbb80", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 30, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 30, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2141062, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_030.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 120, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-31": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 11, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e00cab06-eee1-4712-b515-cead2cd196f2", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 31, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 31, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2141509, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_031.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 121, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:43 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-32": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 12, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "a8f78f5a-56d5-43f4-acbc-5484bfdef79e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 32, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 32, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2141925, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_032.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 122, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-33": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 13, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "ece29f3b-9976-4fa5-9f1b-76b7c5d1210a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 33, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 33, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2142372, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_033.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 123, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-34": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 14, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "88aba775-db43-4b7f-a6de-d1f482db405a", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 34, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 34, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2142806, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_034.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 124, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:44 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-35": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 15, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "e688c870-4915-4f1a-a624-f20a438924f5", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 35, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 35, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2143236, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_035.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 125, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-36": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 16, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "d360798d-b69f-4bdb-8905-9f4bc986cfab", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 36, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 36, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2143685, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_036.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 126, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:45 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-37": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 17, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "9bf2592f-88a1-4a0b-ba95-972e21755206", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 37, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 37, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2144132, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_037.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 127, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-38": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 18, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "57925962-f666-4cd2-bd80-346b003701a0", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 38, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 38, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2144572, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_038.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 128, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:46 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-39": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 19, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "62a26587-224a-4ab3-9ffb-c6f57d96d98d", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 39, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 39, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2144995, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_039.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 129, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" }, "FrameKey-0-4-40": { "Objective-Name": "Objective", "Condenser-Name": "Condenser", "Core-Initialize": "1", "405-DA Device": "405 Shutter", "470 Shutter-Block voltage": "false", "555-Name": "DA Shutter", "640 AO-Demo": "No", "405 Shutter-MaxVolts": "5.0000", "ManualFocus-Name": "ManualFocus", "470-Name": "DA Shutter", "520 AO-Volts": "0.0000", "ROI": "0-0-2048-2048", "520 AO-Description": "NI DAC", "Prime BSI-PP 2 THRESHOLD": "120", "Prime BSI-Port": "CMOS", "555 Shutter-MaxVolts": "5.0000", "470 AO-Description": "NI DAC", "470-State": "0", "LudlShutter-Description": "Ludl shutter adapter", "Prime BSI-PP 5 NO OF ITERATIONS": "2", "SlicePosition": 20, "Prime BSI-CCDTemperatureSetPoint": "-20.0000", "640 Shutter-TriggerSampleRate": "100000", "Exposure-ms": 10, "405 AO-MaxVolts": "5.0000", "COM6-DelayBetweenCharsMs": "0.0000", "Prime BSI-PP 4 DENOISING": "DENOISING", "640-Description": "DA device that is used as a shutter", "445 Shutter-Volts": "0.0000", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "640 AO-Sequenceable": "1", "405-Description": "DA device that is used as a shutter", "445-DA Device": "445 Shutter", "PI AO-Demo": "No", "470 AO-MinVolts": "0.0000", "LudlShutter-LudlShutterNumber": "1", "555 Shutter-MinVolts": "0.0000", "Prime BSI-FanSpeedSetpoint": "High", "405 AO-MinVolts": "0.0000", "AxisPositions": null, "DA Z Stage-Stage Low Voltage": "0.0000", "Prime BSI-Name": "PMPCIECam00", "Prime BSI-FTCapable": "No", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "520 AO-TriggerInputLine": "/Dev1/PFI0", "PI AO-TriggerSequenceLength": "1000", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "XYStage-Port": "COM4", "XYStage-MaxSpeed": "100", "445 Shutter-IOChannel": "Dev1/ao8", "Prime BSI-PP 5 QUANTVIEW": "QUANTVIEW", "Prime BSI-CCDTemperature": "-19.7600", "Prime BSI-SMARTStreamingEnabled": "No", "Objective-ClosedPosition": "", "640 Shutter-IOChannel": "Dev1/ao13", "520 AO-TriggerSequenceLength": "1000", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-ShutterMode": "Pre-Exposure", "445-Description": "DA device that is used as a shutter", "445-Name": "DA Shutter", "COM3-Handshaking": "Off", "405 AO-Description": "NI DAC", "520-State": "0", "UUID": "484afc3c-de2a-4a29-a65f-8f91603ad70e", "Core-Camera": "Prime BSI", "Core-SLM": "", "528 AO-Block voltage": "false", "470 Shutter-MinVolts": "0.0000", "XLIGHT Disk slider-State": "1", "Core-ChannelGroup": "Channels1", "PositionIndex": 22, "WaitInterval": null, "XLIGHT Disk slider-Label": "Disk Pos50um", "DA Z Stage-Name": "DA Z Stage", "555-DA Device": "555 Shutter", "Prime BSI-X-dimension": "2048", "470 AO-MaxVolts": "5.0000", "445 Shutter-Demo": "No", "Prime BSI-CircularBufferFrameRecovery": "OFF", "445 AO-TriggerSampleRate": "100000", "Prime BSI-PVCAM Adapter Version": "1.3.39", "528 AO-Description": "NI DAC", "470 Shutter-SupportsTriggering": "0", "528 AO-MinVolts": "0.0000", "528 Shutter-Sequenceable": "1", "470 AO-TriggerSequenceLength": "1000", "520 Shutter-TriggerSequenceLength": "1000", "Prime BSI-PP 4 THRESHOLD": "85", "Summary": { "Slices": 41, "InitialPositionList": [ { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33111.944 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33111.944 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33111.944 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33111.944 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33111.944 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33390.472 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33390.472 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33390.472 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33390.472 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33390.472 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33669 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33669 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33669 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33669 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33669 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 33947.528000000006 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 33947.528000000006 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 33947.528000000006 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 33947.528000000006 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 33947.528000000006 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55659.944, 34226.056000000004 ] }, "GridColumnIndex": 0, "Label": "20-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 55938.472, 34226.056000000004 ] }, "GridColumnIndex": 1, "Label": "20-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56217, 34226.056000000004 ] }, "GridColumnIndex": 2, "Label": "20-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56495.528000000006, 34226.056000000004 ] }, "GridColumnIndex": 3, "Label": "20-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8319.54], "XYStage": [ 56774.056000000004, 34226.056000000004 ] }, "GridColumnIndex": 4, "Label": "20-Pos_004_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33521.944 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33521.944 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33521.944 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33521.944 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33521.944 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_000", "GridRowIndex": 0 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 33800.472 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 33800.472 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 33800.472 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 33800.472 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 33800.472 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_001", "GridRowIndex": 1 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34079 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34079 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34079 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34079 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34079 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_002", "GridRowIndex": 2 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34357.528000000006 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34357.528000000006 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34357.528000000006 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34357.528000000006 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34357.528000000006 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_003", "GridRowIndex": 3 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62030.944, 34636.056000000004 ] }, "GridColumnIndex": 0, "Label": "21-Pos_000_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62309.472, 34636.056000000004 ] }, "GridColumnIndex": 1, "Label": "21-Pos_001_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62588, 34636.056000000004 ] }, "GridColumnIndex": 2, "Label": "21-Pos_002_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 62866.528000000006, 34636.056000000004 ] }, "GridColumnIndex": 3, "Label": "21-Pos_003_004", "GridRowIndex": 4 }, { "DeviceCoordinatesUm": { "ManualFocus": [8320.68], "XYStage": [ 63145.056000000004, 34636.056000000004 ] }, "GridColumnIndex": 4, "Label": "21-Pos_004_004", "GridRowIndex": 4 } ], "Interval_ms": 0, "UUID": "24cb5292-a9f3-4cf7-bfba-4229bb6fb32c", "UserName": "Nikon", "Depth": 2, "PixelType": "GRAY16", "Time": "2019-09-17 13:55:15 -0400", "Date": "2019-09-17", "z-step_um": 1, "MetadataVersion": 10, "PositionIndex": 22, "SlicesFirst": true, "ChContrastMin": [ 0, 0, 0, 0, 0 ], "Width": 2048, "PixelAspect": 1, "MicroManagerVersion": "1.4.23 20190321", "ROI": [ 0, 0, 2048, 2048 ], "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC" ], "IJType": 1, "GridRow": 0, "Comment": "", "Height": 2048, "GridColumn": 0, "Prefix": "seq1-20and21retake_1", "PixelSize_um": 0.16, "Frames": 1, "CameraTimeout": 20000, "BitDepth": 16, "KeepShutterOpenChannels": true, "Source": "Micro-Manager", "Channels": 5, "ComputerName": "ZadorConfocal1", "CustomIntervals_ms": [], "KeepShutterOpenSlices": false, "ChColors": [ -13369345, -256, -52225, -1, -1 ], "TimeFirst": false, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536 ], "Positions": 50, "Directory": "F:\\20190916barseq sample 2" }, "Source": "Prime BSI", "PI AO-SupportsTriggering": "0", "Prime BSI-PVCAM Version": "3.8.0", "XLIGHT Dichroic-Label": "QUAD", "COM4-StopBits": "1", "555 Shutter-TriggerSampleRate": "100000", "Prime BSI-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "445 AO-MaxVolts": "5.0000", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "TransmittedLamp-Description": "", "640 Shutter-Name": "AnalogIO", "FilterTurretOLYMPUS-Name": "FilterCube", "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-MaxVolts": "5.0000", "COM4-DelayBetweenCharsMs": "0.0000", "LightPath-State": "1", "520 Shutter-MaxVolts": "5.0000", "470 Shutter-MaxVolts": "5.0000", "PI AO-MaxVolts": "5.0000", "640 AO-IOChannel": "Dev1/ao6", "555 AO-TriggerSequenceLength": "1000", "Objective-Description": "Olympus Objective Turret", "Prime BSI-PP 3 ENABLED": "Yes", "XLIGHT Emission Wheel-Label": "647/57", "COM3-Parity": "Even", "405-State": "0", "520 Shutter-Volts": "0.0000", "405-Name": "DA Shutter", "DA Z Stage-Stage Low Position(um)": "0.0000", "OlympusHub-Port": "COM3", "Shutter1-Description": "Shutter Number 1", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "LudlShutter-LudlDeviceNumberShutter": "1", "LudlController-Version": "Version no. : 8.400 MAC5000 Interface with USB ", "PI AO-TriggerSampleRate": "100000", "Core-Galvo": "", "Position": "Default", "CameraChannelIndex": 0, "SliceIndex": 40, "Core-Focus": "DA Z Stage", "445 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-SupportsTriggering": "0", "FrameIndex": 0, "COM6-BaudRate": "9600", "405 AO-Demo": "No", "TransmittedLamp-State": "1", "LightPath-ClosedPosition": "", "Condenser-Label": "Position-2", "520 AO-TriggerSampleRate": "100000", "COM4-Parity": "None", "Core-ImageProcessor": "", "520 AO-AutoDetectedOutputPort": "Dev1/ao3", "Prime BSI-Timing-ExposureTimeNs": "199998000.0000", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "Prime BSI-ShutterCloseDelay": "0", "405 AO-Sequenceable": "1", "528 Shutter-Volts": "0.0000", "COM7-Description": "Serial port driver (boost:asio)", "OlympusHub-Version": "V02.01.01,V01.02.01,V01.03.01", "445 Shutter-Block voltage": "false", "Core-AutoShutter": "1", "COM7-DelayBetweenCharsMs": "0.0000", "445 Shutter-SupportsTriggering": "0", "PI AO-Description": "NI DAC", "Prime BSI-Color": "OFF", "TransmittedLamp-ClosedPosition": "", "Core-XYStage": "XYStage", "Prime BSI-PP 3 THRESHOLD": "85", "Shutter1-State": "0", "PI AO-MinVolts": "0.0000", "528 AO-Sequenceable": "1", "528 Shutter-Name": "AnalogIO", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "640 AO-AutoDetectedOutputPort": "Dev1/ao6", "405 Shutter-Description": "NI DAC", "405 Shutter-IOChannel": "Dev1/ao7", "Core-AutoFocus": "", "470 Shutter-Volts": "0.0000", "Objective-State": "0", "640 Shutter-Description": "NI DAC", "640 AO-MinVolts": "0.0000", "BitDepth": 16, "Slice": 40, "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "Prime BSI-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "PI AO-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-TriggerInputLine": "/Dev1/PFI0", "COM4-Verbose": "1", "COM3-Fast USB to Serial": "Enable", "Prime BSI-ClearMode": "Auto", "PI AO-Volts": "2.5000", "Condenser-State": "1", "555 Shutter-SupportsTriggering": "0", "Core-Shutter": "LudlShutter", "528 AO-TriggerSequenceLength": "1000", "XLIGHT Hub-Port": "COM6", "Prime BSI-ActualInterval-ms": "22.9600", "PI AO-IOChannel": "Dev1/ao14", "520 AO-MaxVolts": "5.0000", "520 Shutter-Description": "NI DAC", "ElapsedTime-ms": 2145412, "640 Shutter-Sequenceable": "1", "PI AO-Name": "AnalogIO", "470 Shutter-Description": "NI DAC", "445-State": "0", "Frame": 0, "520-Name": "DA Shutter", "ManualFocus-Description": "Manual Focus (Z-Drive)", "Prime BSI-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "Prime BSI-AcquisitionMethod": "Callbacks", "PositionName": "20-Pos_002_004", "XLIGHT Spin motor-Label": "On (spinning)", "640 AO-TriggerSampleRate": "100000", "520 Shutter-SupportsTriggering": "0", "Prime BSI-CircularBufferEnabled": "ON", "Height": 2048, "555 AO-TriggerInputLine": "/Dev1/PFI0", "470 Shutter-TriggerSampleRate": "100000", "Objective-Use Safe Position": "1", "555 AO-Demo": "No", "LudlController-TransmissionDelay": "0", "COM6-Handshaking": "Off", "Prime BSI-PP 5 LAMBDA": "26", "FilterTurretOLYMPUS-Description": "Fluorescence Filter Cubes", "445 Shutter-Name": "AnalogIO", "COM3-Name": "COM3", "445 Shutter-TriggerSampleRate": "100000", "LudlController-Reset": "Operate", "520 Shutter-Name": "AnalogIO", "470 AO-Name": "AnalogIO", "XLIGHT Hub-1-Description": "XLIGHT hub", "640 Shutter-MinVolts": "0.0000", "Prime BSI-FullWellCapacity": "45000", "COM3-DelayBetweenCharsMs": "0.0000", "PixelType": "GRAY16", "Prime BSI-PP 2 ENABLED": "Yes", "Prime BSI-PP 7 Reset": "No", "640 Shutter-TriggerSequenceLength": "1000", "Prime BSI-ChipName": "GS2020", "Prime BSI-Trigger-Expose Out-Mux": "1", "COM7-Name": "COM7", "528 AO-TriggerInputLine": "/Dev1/PFI0", "640 Shutter-SupportsTriggering": "0", "COM7-DataBits": "8", "Condenser-Description": "Transmitted Light Condenser", "520 AO-Demo": "No", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "445 AO-IOChannel": "Dev1/ao1", "Prime BSI-Timing-PreTriggerDelayNs": "0.0000", "Prime BSI-PP 1 ENABLED": "Yes", "Prime BSI-TransposeMirrorX": "0", "ManualFocus-FarLimit": "0.0100", "Prime BSI-TransposeMirrorY": "0", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "COM6-Name": "COM6", "405 Shutter-Sequenceable": "1", "445 Shutter-MinVolts": "0.0000", "FilterTurretOLYMPUS-ClosedPosition": "", "528 AO-Demo": "No", "COM6-Fast USB to Serial": "Disable", "445 Shutter-TriggerSequenceLength": "1000", "Prime BSI-TransposeCorrection": "0", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao7", "528 Shutter-TriggerSampleRate": "100000", "COM7-AnswerTimeout": "500.0000", "405 AO-Volts": "0.0000", "555 AO-IOChannel": "Dev1/ao5", "COM7-Handshaking": "Off", "528-State": "0", "640-Name": "DA Shutter", "640 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "Prime BSI-Gain": "1-HDR", "FilterTurretOLYMPUS-Label": "Position-6", "405 Shutter-TriggerSequenceLength": "1000", "640 Shutter-Block voltage": "false", "528 Shutter-IOChannel": "Dev1/ao11", "Prime BSI-ClearCycles": "2", "555 Shutter-Demo": "No", "XYStage-StepSizeX_um": "1.0000", "640 AO-Name": "AnalogIO", "Objective-Safe Position": "100.0000", "PI AO-Sequenceable": "1", "470 AO-Sequenceable": "1", "OlympusHub-Model": "IX2", "Prime BSI-Exposure": "10.00", "Prime BSI-ExposeOutMode": "Rolling Shutter", "520 Shutter-MinVolts": "0.0000", "XLIGHT Hub-1-Port": "COM6", "COM6-Parity": "None", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM7-Parity": "None", "640 AO-TriggerSequenceLength": "1000", "520 AO-Sequenceable": "1", "Prime BSI-Description": "PVCAM API device adapter", "405 Shutter-Block voltage": "false", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 AO-TriggerSampleRate": "100000", "Prime BSI-CircularBufferAutoSize": "ON", "Objective-Label": "40x", "555 Shutter-Sequenceable": "1", "640 Shutter-Volts": "0.0000", "LudlController-Name": "LudlController", "ManualFocus-NearLimit": "30000.0000", "555 Shutter-Name": "AnalogIO", "XLIGHT Spin motor-State": "1", "445 AO-Demo": "No", "470 AO-IOChannel": "Dev1/ao2", "Prime BSI-ShutterOpenDelay": "0", "COM7-StopBits": "2", "555-State": "0", "Prime BSI-Offset": "100", "COM3-AnswerTimeout": "500.0000", "520 AO-Name": "AnalogIO", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "555 AO-SupportsTriggering": "0", "COM7-Fast USB to Serial": "Disable", "Prime BSI-PP 2 MIN ADU AFFECTED": "200", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "520 Shutter-Block voltage": "false", "Prime BSI-Timing-PostTriggerDelayNs": "11200.0000", "640-DA Device": "640 Shutter", "640 AO-SupportsTriggering": "0", "470-Description": "DA device that is used as a shutter", "XLIGHT Emission Wheel-State": "3", "528 AO-TriggerSampleRate": "100000", "DA Z Stage-Stage High Voltage": "5.0000", "405 Shutter-Demo": "No", "LightPath-Description": "Light Path Prism", "528 AO-AutoDetectedOutputPort": "Dev1/ao4", "OlympusHub-Description": "Olympus Hub", "520 AO-MinVolts": "0.0000", "Prime BSI-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "PixelSizeUm": 0.16, "528 AO-MaxVolts": "5.0000", "445 Shutter-MaxVolts": "5.0000", "640 AO-Block voltage": "false", "555 AO-Name": "AnalogIO", "528 Shutter-TriggerSequenceLength": "1000", "COM4-DataBits": "8", "520 Shutter-Sequenceable": "1", "405 Shutter-Volts": "0.0000", "COM3-DataBits": "8", "DA Z Stage-Stage High Position(um)": "220.0000", "470 AO-Demo": "No", "555 AO-Sequenceable": "1", "Condenser-ClosedPosition": "", "Prime BSI-ReadoutRate": "100MHz 16bit", "Binning": "1x1", "405 Shutter-SupportsTriggering": "0", "555 AO-Block voltage": "false", "528 AO-SupportsTriggering": "0", "520 Shutter-TriggerSampleRate": "100000", "470 Shutter-Name": "AnalogIO", "Shutter1-Name": "Shutter1", "COM4-Description": "Serial port driver (boost:asio)", "COM3-Description": "Serial port driver (boost:asio)", "520 Shutter-IOChannel": "Dev1/ao10", "Prime BSI-PP 4 ENABLED": "Yes", "Prime BSI-PreampDelay": "0", "405 AO-Block voltage": "false", "405 Shutter-Name": "AnalogIO", "XYStage-TransposeMirrorY": "0", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "XPositionUm": 56217, "XYStage-TransposeMirrorX": "0", "COM6-DataBits": "8", "OlympusHub-Name": "OlympusHub", "470-DA Device": "470 Shutter", "640 AO-TriggerInputLine": "/Dev1/PFI0", "528 Shutter-Block voltage": "false", "FileName": "img_000000000_P-DIC_040.tif", "528 Shutter-Description": "NI DAC", "XYStage-Acceleration": "100", "555 Shutter-Description": "NI DAC", "LightPath-Name": "LightPath", "445 AO-Description": "NI DAC", "470 AO-Volts": "0.0000", "ZPositionUm": 130, "528-DA Device": "528 Shutter", "640 AO-Description": "NI DAC", "Channel": "P-DIC", "XLIGHT Excitation Wheel-Label": "Quad", "470 Shutter-Sequenceable": "1", "528 Shutter-Demo": "No", "405 Shutter-MinVolts": "0.0000", "Prime BSI-CircularBufferFrameCount": "16", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "Prime BSI-PP 5 GAIN": "100", "LightPath-Label": "Side Port", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 Shutter-MaxVolts": "5.0000", "COM6-Description": "Serial port driver (boost:asio)", "555 AO-MinVolts": "0.0000", "640 Shutter-Demo": "No", "DA Z Stage-DA Device": "PI AO", "555 Shutter-TriggerSequenceLength": "1000", "Camera": "", "ManualFocus-FocusSensitivity": "100", "Prime BSI-PMode": "Normal", "445 AO-Sequenceable": "1", "Prime BSI-TransposeXY": "0", "Prime BSI-PP 4 MIN ADU AFFECTED": "200", "520 AO-SupportsTriggering": "0", "XLIGHT Hub-Name": "XLIGHT Hub", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "XYStage-StepSizeY_um": "1.0000", "PI AO-Block voltage": "false", "520 AO-IOChannel": "Dev1/ao3", "Prime BSI-PP 6 ENABLED": "No", "470 Shutter-Demo": "No", "555 AO-MaxVolts": "5.0000", "520-DA Device": "520 Shutter", "LudlShutter-Name": "LudlShutter", "405 AO-Name": "AnalogIO", "405 AO-TriggerSampleRate": "100000", "XLIGHT Dichroic-State": "2", "NextFrame": 0, "ManualFocus-Position": "8518.1100", "528-Description": "DA device that is used as a shutter", "TransmittedLamp-Label": "On", "445 Shutter-Sequenceable": "1", "555 AO-AutoDetectedOutputPort": "Dev1/ao5", "COM6-StopBits": "1", "PI AO-TriggerInputLine": "/Dev1/PFI0", "Time": "2019-09-17 13:56:47 -0400", "405 AO-IOChannel": "Dev1/ao0", "COM7-Verbose": "1", "COM4-Name": "COM4", "COM4-BaudRate": "9600", "445 Shutter-Description": "NI DAC", "640-State": "0", "470 AO-Block voltage": "false", "528 AO-Volts": "0.0000", "640 AO-MaxVolts": "5.0000", "Prime BSI-MetadataEnabled": "No", "445 AO-Block voltage": "false", "520-Description": "DA device that is used as a shutter", "Prime BSI-PreampOffLimit": "10000", "COM6-Verbose": "0", "555 Shutter-Block voltage": "false", "445 AO-TriggerSequenceLength": "1000", "520 AO-Block voltage": "false", "Prime BSI-TriggerMode": "Internal Trigger", "405 AO-TriggerInputLine": "/Dev1/PFI0", "ChannelIndex": 4, "Prime BSI-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "445 AO-MinVolts": "0.0000", "COM7-BaudRate": "9600", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSequenceLength": "1000", "Prime BSI-Timing-ClearingTimeNs": "0.0000", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "Prime BSI-SerialNumber": "A18J204029", "555-Description": "DA device that is used as a shutter", "Prime BSI-Timing-ReadoutTimeNs": "22937000.0000", "Prime BSI-Binning": "1x1", "Prime BSI-PP 1 THRESHOLD": "115", "Width": 2048, "445 AO-SupportsTriggering": "0", "TransmittedLamp-Name": "", "DA Z Stage-Description": "ZStage controlled with voltage provided by a DA board", "470 AO-SupportsTriggering": "0", "COM3-StopBits": "2", "528 Shutter-MinVolts": "0.0000", "COM3-Verbose": "1", "Prime BSI-PP 5 ENABLED": "No", "640 AO-Volts": "0.0000", "FilterTurretOLYMPUS-State": "5", "528-Name": "DA Shutter", "470 Shutter-IOChannel": "Dev1/ao9", "OlympusHub-Control": "Manual + Computer", "520 Shutter-Demo": "No", "XLIGHT Hub-Description": "XLIGHT hub", "555 Shutter-IOChannel": "Dev1/ao12", "XYStage-Description": "Prior XY stage driver adapter", "Prime BSI-PixelType": "16bit", "Prime BSI-Y-dimension": "2048", "Prime BSI-Trigger Timeout (secs)": "10", "555 AO-Description": "NI DAC", "COM4-Handshaking": "Off", "XLIGHT Excitation Wheel-State": "0", "405 AO-SupportsTriggering": "1", "XYStage-SCurve": "100", "528 AO-Name": "AnalogIO", "405 AO-TriggerSequenceLength": "1000", "LudlController-Port": "COM7", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "555 AO-Volts": "0.0000", "405 Shutter-TriggerSampleRate": "100000", "COM4-AnswerTimeout": "500.0000", "YPositionUm": 34226.056000000004, "445 AO-Name": "AnalogIO", "ManualFocus-FocusWheel": "Off", "555 Shutter-Volts": "0.0000", "XLIGHT Hub-1-Name": "XLIGHT Hub", "COM6-AnswerTimeout": "5000.0000", "528 AO-IOChannel": "Dev1/ao4", "LudlShutter-State": "0", "DA Z Stage-Position": "110.0000", "445 AO-Volts": "0.0000", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "470 AO-TriggerSampleRate": "100000", "Prime BSI-PP 5 OFFSET": "25", "TransmittedLamp-Voltage": "6.1000", "COM3-BaudRate": "19200", "Prime BSI-FirmwareVersion": "23.22", "COM4-Fast USB to Serial": "Disable", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao9" } }